《植物生理学报》 2009, 45(8): 761-764

外源Ca²⁺对海水胁迫下油菜生长和某些与耐盐性有关的生理生化指标缓解效应

范美华^1,*, 马玉心¹, 何光源², 石戈¹, 崔大练¹, 郑文婕¹, 谢芳¹

1 浙江海洋学院海洋科学学院, 浙江舟山316004; 2 华中科技大学生命科学与技术学院, 武汉430074

通信作者：范美华；E-mail: dinger503@163.com；Tel: 0580-2550039

摘　要：

采用水培方法研究外源Ca2+对海水胁迫油菜幼苗生长和其叶中抗氧化酶活性的缓解效应的结果表明, 30%的海水显 著抑制油菜幼苗生长; 适宜浓度的外源Ca2+可以有效缓解海水对油菜的胁迫作用, 即油菜种子的发芽率、苗高和鲜重以及 叶中叶绿素含量均增加, 丙二醛(MDA)含量下降, 超氧化物歧化酶(SOD)和过氧化物酶(POD)活性维持较高水平; 较高浓度 的Ca2+ 则抑制油菜幼苗的生长; 缓解海水胁迫油菜的适宜Ca2+ 浓度为10~20 mmol•L-1

关键词：Ca2+; 油菜; 海水胁迫; 缓解

收稿：2009-03-05   修定：2009-05-31

资助：浙江省重大科技专项优先主题国际合作项目(2007C14021)

Mitigative Effect of Exogenous Calcium on Growth States, Physiological and Biochemical Indexes Related to Salt Tolerance of Brassica napus under Seawater Stress

FAN Mei-Hua^1,*, MA Yu-Xin¹, HE Guang-Yuan², SHI Ge¹, CUI Da-Lian¹, ZHENG Wen-Jie¹, XIE Fang¹

¹Marine Science College, Zhejiang Ocean University, Zhoushan, Zhejiang 316004, China; ²College of Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China

Corresponding author: FAN Mei-Hua; E-mail: dinger503@163.com; Tel: 0580-2550039

Abstract:

Mitigative effects of exogenous calcium on the plant growth and leaf anti-oxidative enzyme activities of Brassica napus seedlings under seawater stress were investigated with liquid culture method. The results showed that growth states of Brassica napus were significantly inhibited in 30% seawater culture. Optimum concentration of CaCl₂ could effectively relieve the seawater damage to Brassica napus, resulting in elevated germination rate, seedling height, fresh weight and chlorophyll content; while SOD and POD maintained high activities. The results from this study showed that the proper concentration range for the growth of Brassica napus were from 10 to 20 mmol·L^-1, and excessively high CaCl₂ concentration had inhibition on seedling growth.

Key words: Ca²⁺; Brassica napus; seawater stress; mitigative